A1 Big Dye Clean Up Kit uses gel filtration technology to remove terminator dyes for sequence analysis with spin columns. Sequence reactions are loaded into the filtration material after transfer of the hydrated gel to the spin column. After a short centrifugation step, the reactions are ready to be loaded into a capillary column. Non-entrapment dye spots are retained in the gel matrix. A1 Big Dye Clean Up Kit removes any dye spot from 10-50 μl sequencing reactions, including terminator dyes. After the cleaning process, sorting reactions can be separated by any capillary sequence. A1 Big Dye Cleaning Kit is optimized for quick and easy dye spot removal, resulting in high quality sequencing results. On the contrary, cleaning the sorting reaction with ethanol precipitation is very time consuming and inefficient. Failure to efficiently remove dye spots results in sequential unreadable dye droplets in the array data sequence. Using A1 Big Dye Clean Up Kit for reaction cleaning sequencing ensures that the reactions loaded on the sequencing tools are free from dye spots. Easily readable throughout the sequence without dye spots.