A1 FFPE DNA Isolation Kit is designed for fast and easy purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue. It has the ability to remove paraffin without using xylene with special melt buffer for deparaphization. If desired two methods can be combined paraffin removal can be performed using a xylene and ethanol method or an efficient heat treatment. Samples are incubated in a specialized lysis buffer along with Proteinase K to reverse crosslinking, effectively releasing short and long DNA fragments. After adjusting the binding conditions with ethanol, the lysate is applied to A1 DNA column to bind DNA. Cellular debris and proteins are effectively removed during the wash steps. All process steps are completed in 80 minutes. High quality purified DNA is then eluted in elution buffer or water and is ready for applications such as PCR and next-generation sequencing.
