A1 FFPE RNA Kit is specially designed for isolation of total RNA from formalin-fixed, paraffin- embedded tissue sections. This method can also be used for the preparation of total RNA from fibrous samples such as heart and muscles tissue.
RNases are rendered inactive by heating while deparaffinization is achieved with the special lysis buffer provided with the kit. It is then incubated with Proteinase K to lysate the digestion proteins. The protein and paraffin-free lysate are then applied to an A1 RNA column to capture the RNA. After a rapid wash step, the purified RNA is eluted with RNase-free water. All process steps are completed within 40 minutes. Although A1 FFPE RNA Kit is optimized to minimize the effect of formaldehyde modification, it is not recommended that purified RNA from this kit be used for downstream applications requiring full-length RNA.
|Starting Material||10-50 mg Formalin-Fixed Paraffin-Embedded Tissue|
|Sample Type||Formalin Fixed Paraffin Embedded Tissue|
|Protocol Time||45 minutes|
|Technology||Silica Spin Column|
|Product||Test Size||Catalog No|
|A1 FFPE RNA Isolation Kit||50 Tests||A1-09039050|
|A1 FFPE RNA Isolation Kit||100 Tests||A1-09039100|
|A1 FFPE RNA Isolation Kit||250 Tests||A1-09039250|