A1 miRNA Kit is used for extraction of micro and large RNA from a wide variety of starting materials like cell culture, tissue samples, microorganisms and etc. It’s basically combine the reversible features of silica-based spin column and A1 miRNA lysis buffer to remove cellular artifacts and collect miRNA from the samples. miRNA is separated to allow for quantification applications like PCR, RT-PCR, cloning genotyping and sequencing. The system uses miRNA Lysis Buffer to efficiently lyse cells and protect RNA from degradation. In order to attach RNA to A1 RNA column, a unique non-hazardous organic solvent solution is applied to the sample solution. After the attachment of RNA to the column, an RNase-free elution buffer is passed through the column to obtain miRNA. All process steps are completed in 30 minutes.