A1 Pathogen DNA Kit allows the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of fungi and gram positive and gram negative bacterial species. With the customized lysis buffer, A1 Pathogen DNA isolation kit provides DNA elution of pathogenic organisms from difficult materials such as food, soil, environmental specimens. This kit delivers an inhibitor-free final product.
A1 Pathogen DNA kit uses optimized lysis condition and up to 1 × 109 bacterial cells can be processed for each column. Pathogen cells are grown to log-phase and harvested or harvested directly from the sample. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted, and the sample is applied to a A1 DNA spin column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or Elution Buffer. All process steps are completed in 30 minutes. Purified DNA can be directly used in downstream applications without the need for further purification.
|Starting Material||1 ml culture broth, 200 mg any sample|
|Sample Type||Microorganism Culture Broth, Contaminated Food Sample, Soil Sample, Water Sample, Swap|
|Protocol Time||30 minutes|
|Technology||Silica Spin Column|
|Purity||A 260 / A 280 ratio: 1.80 – 1.90|
|Product||Test Size||Catalog No|
|A1 Pathogen DNA Isolation Kit||50 Tests||A1-09037050|
|A1 Pathogen DNA Isolation Kit||100 Tests||A1-09037100|
|A1 Pathogen DNA Isolation Kit||250 Tests||A1-09037250|